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Table 19: Results of folate contribution by the minor folate compounds in the SPIFAN II samples
obtained by the analysis with and without conjugase treatment. Total folate and Minor Folate
(mcg/100g) calculated as sum of the detected folate compounds as folic acid in reconstitute
beverages/RTF liquid samples. Averages and precision (as % RSD) of replicates analyzed on
separate 3 days (total n=9).
Sample
Matrix
No Conjugase Treatment
Conjugase Treatment
Total
Folates as
FA*
(mcg/100g)
Minor Folate =
Total Folate -
(FA +CH3THF)
mcg/100g
Minor
Folates as
% of Total
Folate
Total Folates
as FA
(mcg/100g)
Minor Folate =
Total Folate -
(FA +CH3THF)
mcg/100g
Minor
Folates as
% of Total
Folate
6
as Beverage
SRM 1849a Infant
/Adult Nutritional
Formula
23.8
<0.4
0.0
25.6
<0.4
0.0
7
as Beverage
Adult Nutritional
Powder Milk
Protein Based
26.3
<0.4
0.0
29.9
<0.4
0.0
8
as Beverage
Infant Formula
powder Partially
Hydrolyzed Milk
Based
13.7
<0.4
0.0
16.1
1.0
6.5
9
as Beverage
Infant Formula
powder Partially
Hydrolyzed Soy
Based
17.2
<0.4
0.0
20.3
2.0
9.7
10
as Beverage
Adult Nutritional
Powder Low Fat
32.6
<0.4
0.0
34.0
<0.4
0.0
11
as Beverage
Child Formula
Powder
17.7
<0.4
0.0
20.4
<0.4
0.0
12
as Beverage
Infant Elemental
Powder
26.4
<0.4
0.0
27.7
<0.4
0.0
13
as Beverage
Infant Formula
powder Milk Based
15.4
<0.4
0.0
17.3
<0.4
0.0
14
as Beverage
Infant Formula
powder Soy Based
16.3
<0.4
0.0
19.5
1.9
9.8
15
as Beverage
Infant Formula RTF
Milk Based (SPIFAN
Control Milk)
15.0
<0.4
0.0
16.5
<0.4
0.0
16
as Beverage
Adult Nutritional
RTF High Protein
47.0
<0.4
0.0
55.4
<0.4
0.0
17
as Beverage
Adult Nutritional
RTF High Fat
57.4
<0.4
0.0
68.1
<0.4
0.0
Minor folate contents in the SPIFAN II samples based on the analysis which did not employ conjugase
treatment during the extraction was found to be negligible and they did not contribute to total folate
found in these samples.
Minor folate contents in the SPIFAN II samples based on the analysis which employed conjugase
during the extraction was found to be negligible in most of the samples. Two of the samples, both of
which are soy based formula were found to have a small amount of minor folate which contributed to
2011.06/Fol-22 (February 2016)
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