AOAC SPIFAN ERP Meeting Book (3-16-16)

Memo

Monique Steegmans

16001n-MSS-MSS-SLV FOS01 AOAC997.08 Beneo-Orafti, 1 Febr 2016, page 2/18

Report: SPIFAN FOS-01 Single Lab Validation (SLV) for fructan in Infant Formula and Adult

Nutritionals.

1. Introduction.

During the September session (2014) at Boca Raton, the SPIFAN FOS/GOS working group

agreed for a Single Lab Validation for the FOS (fructans) determination according to the AOAC

997.08 method. The purpose is to validate the method for infant formula and adult nutritionals.

The AOAC 997.08 method is an enzymatic, chromatographic method for the determination of

fructans in food and feed products. At the time of approval of the AOAC method (1997), a

collaborative study was performed on samples with different matrices. This method is applied on a

daily basis in our lab. The method gives freedom to apply a chromatographic technique as long as

the determination of the sugars can be done accurately and without interference. Depending on

the matrix of the sample, we decide which chromatographic technique is performed, being GC,

HPLC or HPAEC.

For dairy samples our preferred method is GC because of the complexity of the matrix.

The AOAC 997.08 method is on the list of Codex approved methods for fructan determination

(Council directive 90/496/EEC 12 Dec 2012).

A yearly proficiency test is organized by “Deutsche Lebensmittel GbR” DLA in Germany where

different labs are asked to analyze for the fructan content of a double blind commercial sample.

More info can be found on

. The matrix changes every year. Our lab

successfully participates from 2008 on.

2. Methodology

2.1 Samples for SLV.

A Test Materials Kit, ordered at AOAC, and provided by Covance with different products of infant

and adult nutritionals like listed in the table below. As a first step the 3 placebo samples and the 12

fortified samples were screened in duplicate for the presence of fructans.

3 fortified samples (J4G-00019, J4G-00974 and J4G-00980) and also one of the placebo samples

J4G-00013 were found to contain fructans.

The pure products Orafti HP(inulin), Orafti P95(FOS) and Nutraflora P95(FOS) are part of the Test

Materials Kit and were screened in duplicate by 2 different chromatographic techniques, being GC

and HPLC.

The setup of the recovery (spiking) scheme was done in such a way that the 3 different raw

products were used over the whole analytical range.

Fos-01 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE