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Fonterra Co-operative Group
Page 15
Figure 7: Vitamin D
3
MRM chromatogram of milk-based infant formula sample
Figure 8: Vitamin D
2
MRM chromatogram of milk-based infant formula sample
Mass Spectrometry
During method development, product ion scans of the fragmentation of a standard of vitamin D
3
-PTAD and
vitamin D
2
-PTAD [M+H]
+
ions were performed (Figures 9
–
10). Product ions (298 and 280
m/z
) were
identified as being suitable candidates for quantifier and qualifier ions for both vitamin D
2
and vitamin D
3
.
The method was optimised to enhance the signal of the transitions 572.2
Æ
298 and 572.2
Æ
280 for
vitamin D
2
and 560.2
Æ
298 and 560.2
Æ
280 for vitamin D
3
.
VitD-18 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE