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Fonterra Co-operative Group
Page 18
Appendix A: Data—Linearity
Table A1: Data vitamin D
2
linearity experiment 1
Concentration
(1)
NLD
2
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.3
0.04
0.03
0.03
-29.0
1.5
0.18
0.17
0.18
-0.7
7.6
0.90
0.81
0.89
-0.9
15.1
1.80
1.64
1.81
1.0
37.9
4.49
4.06
4.50
0.3
60.6
7.18
6.45
7.17
-0.2
Regression Equation
y = 0.8987x + 0.0105
(1)
Concentration of non-labelled vitamin D
2
in calibration standards
(2)
Ratio of concentration of non-labelled vitamin D
2
to isotope-labelled vitamin D
2
in calibration standards
Table A2: Data vitamin D
2
linearity experiment 2
Concentration
(1)
NLD
2
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.3
0.04
0.03
0.03
-30.1
1.5
0.18
0.15
0.16
-8.5
7.6
0.90
0.78
0.89
-0.4
15.1
1.79
1.58
1.82
1.3
37.8
4.48
3.90
4.51
0.6
60.5
7.18
6.17
7.15
-0.3
Regression Equation
y = 0.8609x + 0.0117
(1)
Concentration of non-labelled vitamin D
2
in calibration standards
(2)
Ratio of concentration of non-labelled vitamin D
2
to isotope-labelled vitamin D
2
in calibration standards
Table A3: Data vitamin D
2
linearity experiment 3
Concentration
(1)
NLD
2
(ng mL
-1
)
Concentration
Ratio
(2)
Peak Area Ratio
Back-calculated
Conc. Ratio
Variance
(%)
0.3
0.03
0.03
0.02
-34.4
1.4
0.17
0.15
0.16
-5.8
7.2
0.86
0.74
0.84
-1.8
14.4
1.71
1.50
1.72
0.6
36.1
4.28
3.77
4.35
1.7
57.8
6.84
5.88
6.80
-0.7
Regression Equation
y = 0.8624x + 0.0137
(1)
Concentration of non-labelled vitamin D
2
in calibration standards
(2)
Ratio of concentration of non-labelled vitamin D
2
to isotope-labelled vitamin D
2
in calibration standards
VitD-18 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE