Candidates for 2016 ERP of the Year

Primary and Secondary Evaluation of Method SJW-11

SJW-11:

Determination of hyperforin in St. John’s wort Extract and Plant Material by High-

performance Liquid Chromatography (INA Method 112.001)

Author(s):

Institute for Nutraceutical Advancement Methods Validation Program

UMMARY OF

ETHOD

ENERAL

OMMENTS

This is a HPLC/UV method used to test the hyperforin content in St. John’s wort. The sample

preparation involves sonication and centrifugation prior to analysis. The method is a fairly typical HPLC

method; however, it does not include any validation data. The majority of well equipped analytical labs

could run this assay.

ROS

TRENGTHS

This is a simple and straightforward HPLC method that most analytical labs would have the analytical

equipment needed to run this method.

ONS

EAKNESSES

The Hyperforin in the solution is not stable. This method does not include any validation data.

EXPERT REVIEW PANEL VOTE

AND

RECOMMENDATION

MOTION:Not to consider this method for First Action Official Method status.

Reif, Schaneberg (Unanimous) Motion Passed

ERP PROFILE SUMMARIES