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of proof of its accuracy. The accuracy of the method can be reduced by potential specific

enzymes (i. e., proline specific endpetidases) which may be present in fermented and

hydrolyzed foods samples. There is a possibility that activities of these types of enzymes may

cause false negative. The manuscript states that 90% of the secalins in rye sour dough was

not detectable by the assay after fermentation. The lack of an alternate method to estimate

secalins in the fermented rye doesn’t allow establishing its true level in the sample. The

secalins were spiked in gluten free quinoa sourdough by fortifying the sample with the

fermented sour dough at the levels so that secalin concentration in spiked samples is

calculated to be 35 and 75 mg/kg. In the absence of true value of secalins in the fermented

sourdough the spike values as well as the spike recoveries calculated in these spike materials

may remain questionable.

ER 6

The accuracy of the method may be affected as the standard uses pepsin-trypsin digested

prolamins, which may be different than the hydrolyzed gluten in foods. The method may

overestimate intact gluten and may not accurately measure gluten in foods containing

mixture of intact and hydrolyzed gluten.

Supporting Data and Information: Does data from collaborative study support the method as written?

ER 1

no

ER 2

No collaborative study protocol was given to the ERP

ER 3

Yes

ER 4

This could be better, but is sufficient

ER 5

The ERP was not consulted in creating protocols for the study. I am not finding those ready

accessible.

ER 6

Yes

Supporting Data and information: Does data collected support the criteria given in the collaborative study

protocol?

ER 1

not as presented. Makes the claim can detect reliably down to the LOQ, but insufficient data

to establish such.

ER 2

yes

ER 3

Yes

ER 4

The SLV/in house study shows the potential strength of the method The AACC collab supports

the method, but also revealed weaknesses / points of attention.

ER 5

Yes. The protocols used in the study to hydrolyze the prolamins may not reflect the process

which take place in the fermented and hydrolyzes samples with respect to the prolamins. But

in the lack of availability of a method or estimation of gluten in fermented and hydrolyzed the

method under review may be valuable.

ER 6

Yes

ERP PROFILE SUMMARIES

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