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ER 5

Satisfactory

ER 6

Method is clearly written I didn't have problems following it, with the exception of the units used for

the enzyme activities. It would be preferable for the authors to define the units of activity for each

enzyme since definitions vary from manufacturer to manufacturer. This will be fundamental if the

enzymes used need to be replaced with others.

ER 7

good

ER 8

Well thought through study and well written

Pros/Strengths

ER 1 Single vessel

ER 2

Relatively more efficient method. Very well studied and validated in SLV. 15 labs. collaboratively

studied the method and analyzed 10 homogenous test materials (animal feeds and pet foods) using the

described method for dietary starch (ranging starch contents of 1-70%). The average within lab.

Repeatability as sr for % Dietary starch was 0.49 with a range of 0.03 to 1.56, and among –laboratory

repeatability of standard deviation sR averaged 0.96 with a range of 0.09 to 2.69. HORRAT averaged 2.0

for all test samples and 1.9 for samples containing dietary starch more than 2%.

ER 3

Measurement of carbohydrates by enzyme-digestion and analysis of the liberated mono-saccharides is

an established approach which has worked well for a range of carbohydrates. The collaborative data

from this study demonstrates this approach works well for dietary starches due to properly accounting

for sucrose & inherent glucose interferences, and in deterring formation of maltulose. Dietary starch is

digested to glucose and the increase in glucose level is used to calculate %dietary starch. Potential

interferences are either accounted for (inherent glucose) or excluded (deter inherent sucrose digestion

and deter maltulose formation).

ER 4

Traditional chemistry that has been well studied. Can be carried out in modestly equipped laboratories

by technical personnel with modest training.

ER 5

Relatively straightforward procedures Satisfactory recovery on glucose and corn starch. Low

interference from sucrose , β-glucan and cellulose. Good repeatability and reproducibility.

ER 6

- A simple method that does not need specialized equipment. - option to use alternative methods for

glucose analysis is mentioned if a lab does not wish to use the GOPOD assay

ER 7

no comment

ER 8

The specific advantages of this method over AOAC Method 996.11 are not clear. With both methods,

good

reproducibility and recovery of starch was obtained over a wide range of samples. This method is no

easier to

perform than 996.11.

Cons/Weaknesses

ER 1 None

ER 2

The method underestimates dietary starch in feeds and foods whose antioxidant content is known to

exceed 10-20 micromol of hydrophilic antioxidant (as ascorbic acid) per 0.1 g of test dry matter. The

method in the current format may not be easily applicable to foods/feeds high in phenolic compounds

(e.g. beets, red sorghum grain).

ERP PROFILE SUMMARIES

274