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Single Lab Validation of a Method for the Determination of β‐
Galactooligosaccharides in Infant Formula & Adult Nutritionals / Sean Austin (NRC,
Lausanne)
25 Jul 2016
CONFIDENTIAL
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Page 14 / 35
3 R E S U L T S
5.1.
System Calibration
A linear model was used to fit the data for calibration purposes (Figure 2) and the model seems to
fit the data well. The relative residuals were calculated and plotted against the analyte
concentration (Figure 3), in general the predicted concentration is within 5 % of the actual although
there are a few outliers, particularly at lower concentrations.
Figure 2: Calibration Curve
Figure 3: Plot of Relative Residuals (%) Against Maltotriose Concentration
y = 6.3654x + 0.0265
R² = 0.9995
0.00
1.00
2.00
3.00
4.00
5.00
6.00
7.00
0.000 0.100 0.200 0.300 0.400 0.500 0.600 0.700 0.800 0.900 1.000
Peak Area Ratio (Maltotriose / Int. Std)
Maltotriose Concentration (µmol/mL)
‐10.0
‐5.0
0.0
5.0
10.0
15.0
20.0
25.0
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00
Difference from actual concentration (%)
Maltotriose Concentration (µmol/mL)
VALIDATION REPORT
FOR ERP USE ONLY
DO NOT DISTRIBUTE