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Saccharomyces cerevisiae
NE095
11
ERCC-00095*
Methanocaldococcus jannaschii
• Designed to challenge nucleic-acid
based detection technologies
• Target DNA sequence inserted via
homologous recombination
- Conveys specificity
- Eliminates false positives from
near-neighbors
- Sequencing confirms one copy
per genome
• Lyophilized format
* External RNA Controls Consortium (ERCC)
- NIST Standard Reference Material (SRM) 2374: DNA Sequence Library for External RNA
Controls
- ERCC-00095 corresponds to the latter three (of eight) open reading frames in the phosphate
specific transport complex component of
M. jannaschii
S. cerevisiae
NE095 in lieu of Biothreat Agents
12
DNA target eliminates
environmental false
positives
Lyophilized yeast can be
crushed into a powder
No false positives in real
events from equipment
contamination
No green light for
threat assay
validation
Low DNA extraction
efficiency, similar to
Bacillus
spores
Minimized real
and perceived risk
Baker’s
yeast
Quantitative material
can track performance
time
Readily available, can
use almost anywhere
BSL-2
BSL-3