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Yeast Incorporation into the Exercise
23
On-site yeast
prep by CSTs
Team assembly
and JIT training
Sample
collection
Plume
model
Decon and chain
of custody
On-site DNA
extraction and qPCR
On-site
results
Sample
Placement
Day 1
Day 2
Day 3
Next – Yeast as a Surrogate Powder
Objective: Establish a protocol
for first responder training in
sample collection and field
assessment using yeast as a
surrogate powder
Part 1: Confirm the limit of
detection for yeast in PHLs
Part 2: Evaluate a protocol for
sampling and detection of
yeast powder
24
Genomic
DNA
Cell
suspension
Spiked
swabs
Potential
RM format
Powder
Processing
DNA
extraction
Detection
Sampling
Example workflow
Data interpretation & reporting
Previous
interlab
New
interlab
9