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Approach – Build on Existing NIST Efforts
Toward a mixed
DNA material
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RM 8375: DNA from 4 microbes
Toward a mixed
cell material
Bioinformatic pipelines to characterize DNA
SRM 2374 for RNA spike-in
mixtures
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Initial
efforts
toward a
mixed
pathogen
DNA RM
NIST
Candidate RM
for whole
yeast cells
Cell quantification
methods for yeast
Standards for Pathogen Identification via Next-
Generation Sequencing (SPIN) Workshop
October 20-21, 2014 at NIST
• Identify measurement challenges and potential solutions associated
with using NGS for pathogen identification
• On the wish list
- DNA, RNA, or cell mixtures at
known ratios
- Guidelines to develop in-house mixtures
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