![Show Menu](styles/mobile-menu.png)
![Page Background](./../common/page-substrates/page0069.jpg)
Approach – Build on Existing NIST Efforts
Toward a mixed
DNA material
29
RM 8375: DNA from 4 microbes
Toward a mixed
cell material
Bioinformatic pipelines to characterize DNA
SRM 2374 for RNA spike-in
mixtures
1 2 3 4 5
6 7 8 9 10
11 12 13 14 15
16 17 18 19 20
21 22 23 24 25
Initial
efforts
toward a
mixed
pathogen
DNA RM
NIST
Candidate RM
for whole
yeast cells
Cell quantification
methods for yeast
Standards for Pathogen Identification via Next-
Generation Sequencing (SPIN) Workshop
October 20-21, 2014 at NIST
• Identify measurement challenges and potential solutions associated
with using NGS for pathogen identification
• On the wish list
- DNA, RNA, or cell mixtures at
known ratios
- Guidelines to develop in-house mixtures
30