70 / 140
NIST-FDA Workshop: Standards for Pathogen
Detection via Next-Generation Sequencing
October 27-28, 2015 at NIST
31
#NISTpathogen
Action item: Develop a mixed
pathogen DNA material
Prototype Mixed Pathogen DNA Material
32
1
2
3
4
5
6
7
8
9 10
11 12 13 14 15
16 17 18 19 20
21 22 23 24 25
Tube Rack – 25 Tubes
Tubes 1-24: Clinically-relevant
microbial pathogens
Tube 25: Human DNA as
“background”
• Genome assembly
• Base level purity (e.g. rare
variants)
• Genomic contaminants
• Quantity (~ 50-100 ng/μL), to
infer genomes per μL
• Stability
Each tube will contain a single
genome and will be characterized: