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The yeast could “serve as a viable resource for the first phase of
training programs” and “be used periodically as a ‘confidence checker’
to allow first responders, under controlled conditions, to review or
practice their skills.”
– APHL Public Health Preparedness and Response (PHPR) Committee
Stakeholder Input on the Use of the Yeast
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Yeast can “provide responders the ability to exercise and evaluate
sample collection… with material that safeguards against the potential
of cross contamination” and “be used as a competency assessment
tool to evaluate the process of sample collection/submission.”
– CPT Bryon Marsh (ret), 4th CST
Prototype Batch of Lyophilized Yeast
•
Homogeneity
– Determine vial-to-vial variability in terms of total cells per vial
and colony forming units (CFUs) per vial
•
Stability –
Assess total and viable cells per vial for real-time and accelerated
test conditions; Also evaluate DNA stability/integrity
•
Fitness for purpose
– Evaluate suitability for end-user
requirements and applications
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Quantity (total cells)
Coulter counter
Hemocytometer
Reference value
Viability (live cells)
Plate counting
Live/dead staining
Insert stability
qPCR
WGS