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© 2015 AOAC INTERNATIONAL
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Forty representative pesticides were selected based on guidance
from the AOAC Method-Centric Committee on Pesticide Residues
to conduct a multilaboratory validation study of the single-
laboratory validated (SLV) method using two representative brands
of tea. The representative pesticides selected for the multilaboratory
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06 06
LOQs for the 653 pesticides included in the SLV ranged from
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A. Principle
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DQG WKH H[WUDFWV DUH FOHDQHG XS ZLWK &OHDQHUW 737 $JHOD
7HFKQRORJLHV 7LDQMLQ &KLQD (QYL&DUE 36$ RU (&36$&%
8&7 %ULVWRO 3$ 86$ %( &DUERQ
J 36$ $JLOHQW
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DUH HOXWHG ZLWK DFHWRQLWULOH±WROXHQH
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dried, dissolved in the recommended solution, and analyzed by
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B. Reagents
(a)
Solvents
.—Acetonitrile, toluene, and
n
KH[DQH +3/&
grade).
(b)
Acetonitrile–toluene.—
Y Y
(c)
Ultrapure water
.—Obtained from a Milli-RO plus system
WRJHWKHU ZLWK D 0LOOL 4 V\VWHP (0' 0LOOLSRUH &RUS %LOOHULFD
MA, USA).
(d)
Anhydrous sodium sulfate
²$QDO\WLFDOO\ SXUH %DNHG
at 650°C for 4 h and stored in a desiccator.
C. Materials
(a)
SPE cartridges
²&OHDQHUW 737
PJ P/ $JHOD
7HFKQRORJLHV RU (QYL&DUE 36$ PJ PJ P/ 6XSHOFR
%HOOHIRQWH 3$ 86$ RU HTXLYDOHQW
(b)
SPE tube adapter
²)RU P/ 63( WXEHV
IRU P/
63( WXEHV
8 6LJPD $OGULFK 6KDQJKDL 7UDGLQJ &R /WG
Shanghai, China), or equivalent.
(c)
'LVSRVDEOH ÀRZ FRQWURO YDOYH OLQHUV
²)RU 9LVLSUHS 70 '/
6LJPD $OGULFK 6KDQJKDL 7UDGLQJ &R /WG
(d)
3HDU VKDSHG ÀDVN
² P/ =
($ 6LJPD $OGULFK
Shanghai Trading Co., Ltd), or equivalent.
See
Figure
2014.09
.
(e)
Reservoir
² P/ $ $JHOD RU HTXLYDOHQW
See
Figure
2014.09
.
(f)
Centrifuge tube
.—80 mL.
(g)
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² PP î ȝP
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