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H
ALL
:
J
OURNAL OF
AOAC I
NTERNATIONAL
V
OL
.
98, N
O
. 2, 2015
397
Determination of Dietary Starch in Animal Feeds and Pet Food
by an Enzymatic-Colorimetric Method: Collaborative Study
M
ARY
B
ETH
H
ALL
U. S. Department of Agriculture–Agricultural Research Service, U.S. Dairy Forage Research Center, 1925 Linden Dr, Madison, WI 53706
Collaborators: J. Arbaugh; K. Binkerd; A. Carlson; T. Doan; T. Grant; C. Heuer; H. D. Inerowicz; B. Jean-Louis; R. Johnson;
J. Jordan; D. Kondratko; E. Maciel; K. McCallum; D. Meyer; C. A. Odijk; A. Parganlija-Ramic; T. Potts; L. Ruiz; S. Snodgrass;
D. Taysom; S. Trupia; B. Steinlicht; D. Welch
Received August 5, 2014.
The method was approved by the Expert Review Panel for Dietary
Starch.
The Expert Review Panel for Dietary Starch invites method users
to provide feedback on the First Action methods. Feedback from
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and are critical to gaining global recognition and acceptance of the
methods. Comments can be sent directly to the corresponding author
or
methodfeedback@aoac.org.Mention of any trademark or proprietary product in this paper does
not constitute a guarantee or warranty of the product by the USDA or
the Agricultural Research Service and does not imply its approval to
the exclusion of other products that also may be suitable.
Corresponding author’s e-mail:
marybeth.hall@ars.usda.govDOI: 10.5740/jaoacint.15-012
FOOD COMPOSITION AND ADDITIVES
Starch, glycogen, maltooligosaccharides, and other
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tarch is an important, frequently analyzed component
of animal feedstuffs. It can have substantial positive
effects on animal performance and potential undesirable
effects on glycemic response and animal health (1). AOAC
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920.40
for starch in animal feeds (2) is no
longer valid because of discontinued production of the enzyme
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in the procedure. Accordingly, another approved method for
starch in animal feeds is needed. Additionally, new terminology
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be used to specify the analysis.
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consisting of long linear unbranched chains of
D
-1,4-linked
D-glucose units (amylose) and/or long
D
-1,6-branched chains
of
D
-1,4-linked glucose units (amylopectin; 3). However, the
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the linkages in plant starch also hydrolyze those same linkages
in glycogen from animal (4) or microbial (5) sources and in
maltooligosaccharides that are breakdown products of starch