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gravimetrically determine the amount of fat eluting in each fraction
by subtracting the empty weights from newly recorded weights
after solvent evaporation. There should be no fat eluting in the
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silica gel cartridge has to be used.
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Analyte
Concentration, μg/mL
Anthracene
10
Benz[
a
]anthracene
10
Benzo[
a
]pyrene
10
Benzo[
b
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10
Benzo[
g,h,i
]perylene
10
Benzo[
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10
Chrysene
25
Dibenz[
a,h
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10
Fluoranthene
25
Fluorene
10
Indeno[1,2,3-
cd
]pyrene
10
Naphthalene
50
Phenanthrene
25
Pyrene
25
1-Methylnaphthalene
25
2,6-Dimethylnaphthalene
25
1-Methylphenanthrene
25
1,7-Dimethylphenanthrene
10
3-Methylchrysene
25
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Calibration
level
Vol. of mixed
stock standard
solution, μL
Vol. of working
PAH solution
a
,
μL
Vol. of working
PAH
solution B, μL
Vol. of
13
C-PAH
1 μg/mL solution,
μL
Final
vol.
a
, μL
1
—
—
50
50
1000
2
—
—
100
50
1000
3
—
—
200
50
1000
4
—
—
500
50
1000
5
—
100
—
50
1000
6
—
200
—
50
1000
7
—
500
—
50
1000
8
100
—
—
50
1000
a
Bring to volume using isooctane.
G. GC/MS Analysis
a
GC conditions.
—Table
2014.08G
provides GC conditions
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be optimized to enable quantitative transfer of less volatile PAHs.
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volatile PAHs, especially naphthalene. The separation criteria
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of benzo[
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Note
: Criteria for separation of chrysene and
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collaborative study. For accurate quantitation of chrysene, at least
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using selective stationary phases.
The maximum oven temperature program may not exceed the
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for their better temperature stability and also good selectivity for
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maintenance to ensure adequate operation of the GC instrument.
Perform system checks.
b
MS conditions.
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