38 / 194
Family of large, double
stranded DNA viruses
Within genera, antigenic similarity:
t ti
Background: Poxvirus 101
cross pro ec on
Complex viruses, cytoplasmic
lifecycle
Genus
Orthopoxvirus:
90-98% nucleotide identity across
species
V i l V i i C
ar o a, acc n a, owpox,
Monkeypox
– all can cause human
disease
•
Variola
evolved to be a sole human
pathogen: SMALLPOX
Camelpox, Ectromelia, Taterapox
are
NOT known to cause human disease
Objectives
• Smallpox introduction
• Development of smallpox clinical diagnostics
• Developing a framework for considering
environmental smallpox detection/testing
– Results of 2016 Standard Method Performance
Requirements for Detection of
Variola virus
DNA in
collection buffers from aerosol collection devices
for DoD applications