33 / 258
1588
H
aselberger
&
J
acobs
:
J
ournal of
AOAC I
nternational
V
ol
. 99, N
o
. 6, 2016
Conclusions
Performance metrics from the validation work indicate that
the method conforms to the range, LOQ, precision, and recovery
requirements established in SMPR 2014.002. In addition,
specificity appears to be adequate, as evidenced by the failure
to detect any apparent fructan in the unfortified samples that
were analyzed. Common carbohydrate ingredients, GOS and
various maltodextrins, although producing more complicated
chromatograms for interpretation, do not generally create undue
difficulty for assignment of CFs in the qualitative analysis. We
conclude that the method is suitable for determination of inulin-
type fructans in infant formulas and pediatric/adult nutritional
products.
Acknowledgments
The authors wish to thank Stephanie (Williams) Neu, Bao
Kou Thao, Ashely (Johnson) Bonavia,
David Ellingson, Brent
Rozema, and Vanessa Spencer
from Covance Laboratories
(Madison, WI) and Ding Yi from Abbott Nutrition, Singapore,
for their efforts in setting up themethodology in their laboratories
and generating results for the Abbott in-house samples listed in
Table
2016.06F
.
References
(1)
Official Methods of Analysis
(2016) 20th Ed., AOAC
INTERNATIONAL, Rockville, MD, SMPR 2014.002. www.
eoma.aoac.org(2) Joye, D., & Hoebregs H. (2000)
J. AOAC Int
.
83
, 1020–1026
(3) De Leenheer, L., & Hoebregs, H. (1994)
Starch – Stärke
46
,
193–196
(4) Durgnat, J., & Martinez, C. (1997)
Seminars in Food Analysis
2
, 85–97
(5) L’homme, C., Peschet, J.L., Puigserver, A., & Biagini, A. (2001)
J. Chromatogr. A
920
, 291–297
(6) Hoebregs, H. (1997)
J. AOAC Int
.
80
, 1029–1037
(7) McCleary, B.V., Murphy, A., & Mugford, D.C. (2000)
J. AOAC
Int
.
83
, 356–364
(8)
Stöber, P., Bénet, S., & Hischenhuber, C. (2004)
J. Agric. Food
Chem
.
52
, 2137–2146
(9) Steegmans, M., Iliaens, S., & Hoebregs, H. (2004)
J. AOAC Int.
87
, 1200–1207
(10) Cuany, D., Bénet, T., & Austin, S. (2010)
J. AOAC Int.
93
,
202–212
Table 3. Data for evaluating LOQ
Sample type
No. of replicates Mean (g/100 g, RTF)
SD
r
RSD
r
, % SD
IP
RSD
IP
, %
0.03% FOS spike in 9% sucrose solution
a
9
b
0.029
0.0018
6.12
0.0021
7.42
Infant formula powder with FOS/GOS
c
12
d
0.036
0.0008
2.14
0.0012
3.29
a
No additional matrix, solution in water.
b
Triplicates on each of 3 days.
c
Material from SPIFAN sample kit.
d
Duplicates on each of 6 days.
33