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H
aselberger
&
J
acobs
:
J
ournal of
AOAC I
nternational
V
ol
.
99, N
o
. 6, 2016
1587
calculation of the actual fructan content but, at the same time,
serves as the primary determinant of selectivity.
LOQ
Data relevant to LOQ is summarized in Table 3. For the
9% sucrose solution spiked with 0.03% scFOS, average
recovery was 97%, RSD
r
was 6.12%, and RSD
IP
was 7.42%.
This RSD
r
at this level is just beyond the SMPR limit of 6%.
Table 3 also shows data for the SPIFAN infant formula powder
with FOS/GOS. The total fructan content of this material was
determined to be very close to the SMPR specified LOQ of
0.03%. For that sample, both RSD
r
(2.14%) and RSD
IP
(3.19%)
were well below the 6% repeatability limit.
Figure 4. Example of product containing Synergy 1 spiked at ~1.1 g/100 g (Part I), – Upper trace: inulin (Beneo Orafti HP); middle trace:
SPIFAN adult nutritional powder low fat, 00859RF00; lower trace: Ingredion Nutraflora scFOS. Time interval from 36–44 min zoomed in to show
detail.
Figure 5. Example of product containing HP (Part I), spiked at ~1.1 g/100 g – Upper trace: inulin (Beneo Orafti HP); middle trace: SPIFAN
infant formula powder partially hydrolyzed soy, 410457651Z; lower trace: Ingredion Nutraflora scFOS. Time interval from 36–44 min zoomed in
to show detail.
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