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H
ostetler
:
J
ournal of
AOAC I
nternational
V
ol
.
100, N
o
.
3, 2017
accuracy was assessed by spike and recovery of all-
trans
-lutein
and β-carotene in various matrixes. Spiking was done with
either FloraGlo 5% lutein (DSM Nutritional Products, Basel,
Switzerland), which was first dispersed in water and added
to the sample in place of water, or with diluted standards
added to the ISTD. Spiked and unspiked samples were
measured in triplicate. Tables 11–17 show that recovery from
spiked samples ranged from 92.3 to 105.5% for lutein and
from 100.1 to 107.5% for β-carotene. The SMPR calls for
90–110% recovery from spiked samples, and all data met this
requirement.
Conclusions
The data presented here were reviewed by the AOAC
Expert Review Panel on SPIFAN Nutrient Methods at the AOAC
INTERNATIONAL Annual Meeting in Dallas, TX, held in
September 2016. The panel found that the data met the criteria
Table 10. Precision data for β-carotene in SPIFAN matrixes and select additional matrixes
a
Formula
Code
n
cis
-BC,
μg/100 g
b
RSD
r
, % RSD
iR
, %
trans
-BC,
μg/100 g RSD
r
, % RSD
iR
, %
Total BC,
μg/100 g RSD
r
, % RSD
iR
, %
Child Formula Powder,
Placebo
00847RF00 3
0.3
0.7
NA
c
0.6
2.6
NA 0.9
1.8
NA
Infant Elemental Powder,
Placebo
00796RF 3
<
d
NA
NA
<
NA NA
<
NA NA
AN High-Protein RTF,
Placebo
e
00821RF00 3
<
NA
NA
0.8
11.2
NA 0.8
11.2
NA
AN High-Fat RTF, Placebo 00820RF00 3
<
NA
NA
0.4
47.4
NA 0.6
48.5
NA
IF Milk-Based RTF, Placebo
f
EV4H2Q 3
0.5
3.3
NA
0.4
2.2
NA 0.9
2.6
NA
SRM 1849a Powder
CLC10-B 3
<
NA
NA
<
NA NA
<
NA NA
IF Milk-Based Partially
Hydrolyzed Powder
410057652Z 3
0.8
0.2
NA
1.0
2.0
NA 1.8
1.2
NA
IF Soy-Based Partially
Hydrolyzed Powder
410057651Z 3
0.6
1.8
NA
0.6
6.7
NA 1.3
3.9
NA
Toddler Formula
Milk-Based Powder
4052755861 12
6.5
1.0
2.4
7.4
0.7
2.9
13.9
0.8
2.2
IF Milk-Based Powder
4044755861 12
4.4
2.1
2.7
10.1
1.3
2.7
14.4
1.5
2.4
AN Powder
00859RF00 3
<
NA
NA
0.3
5.9
NA 0.3
5.9
NA
Child Formula Powder
00866RF00 3
0.3
15.3
NA
0.4
2.5
NA 0.7
5.4
NA
IF Elemental Powder
00795RF 12
7.3
0.3
0.7
15.3
0.7
0.7
22.7
0.5
0.6
IF with FOS/GOS Powder
g
50350017W1 12
1.3
5.1
6.3
3.4
3.0
4.6
4.8
2.3
3.8
IF Milk-Based Powder
(control)
K16NTAV 39
6.0
1.1
2.7
13.2
0.5
2.2
19.2
0.6
1.7
IF Soy-Based Powder
E10NWZC 12
6.5
2.6
2.3
11.0
2.0
1.8
17.5
2.2
1.9
IF Milk-Based RTF
EV4H2R 3
0.6
6.9
NA
0.4
4.8
NA 1.0
4.6
NA
AN High-Protein RTF
00730RF00 12 265
1.6
2.7
534
1.7
2.3
799
1.6
2.4
AN High-Fat RTF
00729RF00 3
<
NA
NA
0.3
7.7
NA 0.4
5.9
NA
AN High-Lutein Powder
—
h
3
<
NA
NA
0.5
18.3
NA 0.5
18.3
NA
Chocolate Pediatric
Nutritional RTF
— 3
<
NA
NA
<
NA NA
<
NA NA
IF RTF
— 3
9.1
0.3
NA
16.4
0.4
NA 25.5
0.4
NA
a
For powder samples, results were calculated using a factor of 25 g powder/225 g reconstituted weight.
b
BC = β-Carotene.
c
NA = Not applicable. RSDr not calculated for concentrations <LOQ; RSDiR not calculated if samples were all prepared on the same day.
d
< = Below the LOQ.
e
AN = Adult Nutritional.
f
IF = Infant Formula.
g
FOS/GOS = Fructooligosaccharide/galactooligosaccharide.
h
— = Sample was sourced locally and was not part of the SPIFAN kit.
Table 11. Spike and recovery data for NIST SRM 1849a
a
Analyte
Native level, μg/100 g
Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
ND
b
1.4
105.5
0.8
13.8
99.6
0.2
β-Carotene
0.2
1.3
107.5
0.9
13.6
103.2
0.3
a
Samples were spiked with standards at the internal standard step of sample preparation.
b
ND = Not detected.
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