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ostetler
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J
ournal of
AOAC I
nternational
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ol
.
100, N
o
.
3, 2017
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for specific carotenoids, (lutein and β-carotene) as stated in SMPR
2014.014, and recommended the method’s approval as an AOAC
First Action method. It is codified as
Official Method
2016.13
.
Acknowledgments
I thank Shang-Jing Pan and Douglas Gordon of Abbott
Nutrition (Columbus, OH) for reviewing the method and
providing feedback, and Steve Tennyson and Scott Christiansen
of Perrigo Nutritionals (Georgia, VT) for reviewing the method
and validation data.
References
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Table 12. Spike and recovery data for NIST SRM 1849a
a
Analyte
Native level,
μg/100 g
Spike,
μg/100 g Recovery, % RSD, %
Spike,
μg/100 g
Recovery, % RSD, %
Spike,
μg/100 g Recovery, % RSD, %
Lutein
ND
b
34.8
92.3
0.8
68.3
94.0
1.9
135.3
97.6
1.6
a
Samples were spiked with FloraGlo 5% lutein at the powder reconstitution step of sample preparation.
b
ND = Not detected.
Table 13. Spike and recovery data for AN High-Protein RTF, Placebo
a
Analyte
Native level,
μg/100 g
Spike,
μg/100 g Recovery, % RSD, %
Spike,
μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
3.3
62.7
96.7
0.8
148.8
100.1
0.3
297.6
101.0
0.3
a
Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step.
Table 14. Spike and recovery data for AN High-Protein RTF, Placebo
a
Analyte
Native level,
μg/100 g
Spike,
μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
3.3
486.4
101.1
0.5
708.8
101.2
0.5
1425.3
100.7
0.2
a
Samples were spiked with FloraGlo 5% lutein at the initial sample dilution step.
Table 15. Spike and recovery data for Child Formula Powder, Placebo
a
Analyte
Native level, μg/100 g Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
0.1
5.5
101.8
0.2
16.8
100.8
0.3
β-Carotene
0.6
5.3
107.2
0.2
16.2
104.2
0.3
a
Samples were spiked with standards at the internal standard step of sample preparation.
Table 16. Spike and recovery data for Infant Elemental Powder, Placebo
a
Analyte
Native level, μg/100 g
Spike, μg/100 g
Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
ND
b
5.5
103.7
0.2
16.8
101.9
0.6
β-Carotene
0.1
5.3
104.1
0.2
16.2
103.5
0.6
a
Samples were spiked with standards at the internal standard step of sample preparation.
b
ND = Not detected.
Table 17. Spike and recovery data for Infant Formula RTF
a
Analyte
Native level, μg/100 g
Spike, μg/100 g Recovery, % RSD, % Spike, μg/100 g Recovery, % RSD, %
Lutein
3.9
6.2
98.7
0.4
18.4
98.6
0.1
β-Carotene
16.4
5.9
100.6
0.9
17.7
100.1
0.6
a
Samples were spiked with standards at the internal standard step of sample preparation.
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