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B
runt
et al
.:
J
ournal of
aoaC I
nternatIonal
V
ol
.
100, n
o
.
3, 2017
11
CCC used the sample Infant Formula Powder, Partially
Hydrolyzed Milk-Based, Lot No. 410057652Z (Table
2016.14A
, sample No. 7) for the determination of the LOD
and LOQ by spiking it with a low level of fructan (Orafti
P95, 0.046 g/100 g) and analyzing the sample 10 times on
2 different days.
The LOD and LOQ were then calculated as
= ×
LOD 3 SD
= ×
LOQ 10 SD
The LOD and LOQ results are summarized in Table 1 and
also meet the requirements outlined in the SMPRs (5). The
established values of the LOD and LOQ of both NRC and CCC
are in good agreement and meet the SMPRs (5).
Precision
It was known that only a few samples in the SPIFAN sample
kit contained fructan. To establish which samples contained
fructan, both NRC and CCC analyzed the whole series of 19
samples independently from each other. In both laboratories, six
of the 19 samples were found to contain fructans:
(1)
Sample No. 1, Child Formula Powder, Lot No. 00847RF00.
(
2
) Sample No. 9, Toddler Formula Powder, Milk-Based,
Lot No. 4052755861.
Figure 3. Calibration curves using chitobiose as internal standard (I.S.; left column) and plots of the relative residuals (right column).
(A) Fructose (Fru) at NRC; (B) glucose (Glc) at NRC; (C) fructose at CCC; and (D) glucose at CCC.
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