AOAC SPIFAN Final Review Forms (March 16, 2016)

FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method:

Fol-20 #1

Title:

Analysis of Folic acid and 5-Methyltetrahydrofolate in Infant and Adult Nutritional formula using Ultra-

Performance Liquid Chromatography-Tandem Mass Spectrometry

Author:

University New South Wales

Summary of Method:

Folate extraction involved treatment in a boiling water bath, solid phase extraction and ultra‐filtration.

Quantitation of folic acid and 5‐methyltetrahysdrofolate is done by ultra‐performance liquid

chromatography‐tandem mass spectrometry (UPLC‐MS/MS).

Method Scope/Applicability:

Infant Formula and Adult/Pediatric Formulas

General comments about the method:

This method is unchanged since previous review by ERP. Previous concern expressed at higher results

obtained from analysis without tri-enzyme compared to results using tri-enzyme digestion.

Method Clarity:

Clear directions for operation of the method, instruments. Clear equations describing how the standards

and samples are quantitated is required.

Method Safety Concerns:

Pros/Strengths:

Cons/Weaknesses

Supporting Data

No new data since last review.

Performance Characteristics:

Analytical Range:

LOQ:

Accuracy/Recovery:

Fol-20 Review Forms