100 / 159
FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method:
Chlor-03/Fluor-02 #
3
Title:
Ion Chromatography method for the determination of Fluoride and Chloride in Infant Formula and Adult
Nutritionals
Author:
Thermofisher
Summary of Method:
Ultra-centrifugation is used to filter the sample and remove the fats and proteins from the sample.
Following the filtration, solution was passed through cation exchange cartridge to remove heavy metals
(such as Iron) from the samples. Samples were then separated using an anion-exchange column and
detected by suppressed conductivity.
Method Scope/Applicability:
Infant Formula and Adult/Pediatric Formulas
General comments about the method:
Method Clarity:
Clear directions for operation of the method, instruments. Clear equations describing how the standards
and samples are quantitated is required.
Method Safety Concerns:
None other than standard laboratory safety
Pros/Strengths:
Very simple straightforward analysis.
Cons/Weaknesses
Unclear as to whether only Thermofisher columns and cartridges are required or if alternatives are
available.
Supporting Data
SPIFAN kit has been used for both fluoride and chloride validation.
Performance Characteristics:
Analytical Range:
4-800 μg/100g fluoride, meets SMPR
4-2400 μg/100g chloride, meets SMPR
Chlor-03/Fluor-02 Review Forms