98 / 159
FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method: Chlor-03 / Fluor-02
Title:
Determination of chloride and fluoride in infant formula and adult nutritionals:
Author:
Thermo Scientific
Reviewer Name:
Summary of Method:
The application describes a procedure for the determination of chloride and fluoride by ion chromato-
graphy with suppressed conductivity measurement. Sample preparation: 2,5 g sample is weighted in a
20 ml polypropylene tube and filled up to 20 ml with demin. Water. X g of demin. water is added and the
weight is recorded. After shaken well for shaken for 2 – 3 minutes 12 ml are transferred into an Amicon
Ultra-15 centrifugal filter device and centrifuged for 60 min at 5000 rpm. 5 ml of the filtrate are passed
through an onguard cartridge, 3 ml are discarded and the remaining 2 ml are filtered through 0,2 µm
before analysis The determination of chloride and fluoride are performed by ion chromatography with
suppressed conductivity measurement.
Method Scope/Applicability:
The method is applicable for the determination of chloride (and fluoride) in IMF and Adult Nutritionals
(shown in single lab validation report)
General comments about the method:
The test sample preparation is easy to perform and does not require special glassware or equipment. The
used ion chromatograph requires some experience of the analyst. The used chromatography columns
IonPac 15 is a protected trademark (functional group: alkanol quaternary ammonium salt).
Method Clarity:
The method is clearly described and easy to follow.
One remark:
Step 2: Add ?g of DI water and record the total weight. According the SMPR the reconstitution of the
powder should be 25g in 200g deionized water). In the method it is not clear described how much water
should added to which total weight.
Pros/Strengths:
Simple sample preparation
Simultaneous determination of chloride and fluoride is prescribed
Method also applicable for other food matrices
#2
Chlor-03/Fluor-02 Review Forms