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FOR EXPERT REVIEW PANEL USE ONLY

NOT FOR DISTRIBUTION

1

Expert Review Panel for Infant Formula and Adult Nutrition

Evaluation of Method: Chlor-03 / Fluor-02

Title:

Determination of chloride and fluoride in infant formula and adult nutritionals:

Author:

Thermo Scientific

Reviewer Name:

Summary of Method:

The application describes a procedure for the determination of chloride and fluoride by ion chromato-

graphy with suppressed conductivity measurement. Sample preparation: 2,5 g sample is weighted in a

20 ml polypropylene tube and filled up to 20 ml with demin. Water. X g of demin. water is added and the

weight is recorded. After shaken well for shaken for 2 – 3 minutes 12 ml are transferred into an Amicon

Ultra-15 centrifugal filter device and centrifuged for 60 min at 5000 rpm. 5 ml of the filtrate are passed

through an onguard cartridge, 3 ml are discarded and the remaining 2 ml are filtered through 0,2 µm

before analysis The determination of chloride and fluoride are performed by ion chromatography with

suppressed conductivity measurement.

Method Scope/Applicability:

The method is applicable for the determination of chloride (and fluoride) in IMF and Adult Nutritionals

(shown in single lab validation report)

General comments about the method:

The test sample preparation is easy to perform and does not require special glassware or equipment. The

used ion chromatograph requires some experience of the analyst. The used chromatography columns

IonPac 15 is a protected trademark (functional group: alkanol quaternary ammonium salt).

Method Clarity:

The method is clearly described and easy to follow.

One remark:

Step 2: Add ?g of DI water and record the total weight. According the SMPR the reconstitution of the

powder should be 25g in 200g deionized water). In the method it is not clear described how much water

should added to which total weight.

Pros/Strengths:

Simple sample preparation

Simultaneous determination of chloride and fluoride is prescribed

Method also applicable for other food matrices

#2

Chlor-03/Fluor-02 Review Forms