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FOR EXPERT REVIEW PANEL USE ONLY
NOT FOR DISTRIBUTION
1
Expert Review Panel for Infant Formula and Adult Nutrition
Evaluation of Method:
Amino-03
#3
Title:
Amino Acids in Infant Formula and Adult/Pediatric Formulas
Author:
TUV/SUD
Summary of Method:
Proteins hydrolysis followed by derivatization using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
(AQC) reagent. The derivatized forms of amino acids are separated by UHPLC and quantitated by Diode
Array Detector (Tryptophan by Fluorescence detector) using Norvaline as internal standard.
Method Scope/Applicability:
Infant Formula and Adult/Pediatric Formulas
General comments about the method:
Standard technique for analyzing amino acids using proprietary AccQ Tag derivatisation followed by LC-UV
analysis.
Method Clarity:
Clear directions for operation of the method, instruments and calculations
Method Safety Concerns:
None other than standard laboratory safety
Pros/Strengths:
Very well established technique for amino acids analysis
Cons/Weaknesses
Use of proprietary AccTag reagents
Supporting Data
Values obtained for NIST 1849a are acceptable against certified values.
No SPIFAN kit used in the validation of the method.
Performance Characteristics:
Analytical Range:
Not specified
LOQ:
Amino-03 Review Forms