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Proposal for analytes:
1. Limit to only principal isomers of the four most
relevant, co‐chromatographing compounds
a. Lutein
b. 3’‐Epilutein (significant epimer loss of lutein)
c. Zeaxanthin
d β‐Cryptoxanthin
.
2. Saponify initial extract
a. Analyze free compounds only
1. Lutein
o
(3R 3’R 6’R) β ε carotene 3 3’ diol
;
dietary
,
,
‐ , ‐
‐ , ‐
o
Commercial and supplemental roles
•
Accumulates throughout human retina
•
Reportedly rescues AMD
•
Present in other tissues, relevance under study
•
Colors white egg yolks yellow
•
Antioxidant
•
Colorant (E161b)
o
Structure
o
Proposed daily dose: 10 mg