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3D Cell Culture
A more physiologically relevant model than 2D assays due to the presence of hypoxia, gradients of
nutrients and increased cell-cell contact
Cells added to ultra-low attachment plates form a 3D spherical structure (‘spheroid’)
Automated imaging and analysis of spheroid growth following radiation was performed with a
Celigo
cytometer
Cell viability was also measured using the Cell Titre Glo
assay (measures ATP production)
500
µm