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3 human cell lines:
- MRC-5, normal lung fibroblasts
- A549, lung cancer, p53 wild-type
- NCL-H23, lung cancer, mutant p53
Irradiation:
- broadbeam
- microbeam
2D clonogenic assays:
- conventional measure of cell sensitivity to radiation
3D cell culture assays:
- spheroid growth curves
- cell viability assay
- image changes in spheroid morphology
Methodology