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AOAC SPDS ERP - Set 3 Review Form 2
Name
Linda Monaci
Organization
CNR-ISPA
Title of Method
Detection and Quantitation of Selected Food
Allergens by LCMS/MS
AOAC Candidate
Method Number
(e.g. ALN-01)
ALL-01
Applicable SMPR
yes
Summary:
The method described is a LC-MS/MS method for
the simultaneous detection of multiple allergens
basing on the detection of unique peptide markers
for each allergenic food namely egg, milk, peanut
and hazelnut along the same LC-MS run. The
method has been run on different triple quadrupole
MS instrument by building an acquisition method
monitoring at least two transitions for each peptide
marker targeted. The sensitivity, specificity and
applicability of the method has been also
investigated in the different food commodities
analysed.
I.
Review
of
Method
Only
1. Does the
applicability of the
method support the
applicability of the
SMPR? If not,
please explain what
is missing.
Yes
2. Does the
analytical
technique(s) used in
the method meet the
SMPR? If not,
please specify how it
Despite the analytical technique that in principle is
expected to meet the SMPR requirements, there is
still an incorrect way of calculating MQL and
MDL. This arises from an erroneous noise
estimation and consequently an erroneous
calculation of the resulting S/N. Despite the correct
approach the authors stick with the use of a