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statements in the
method?
2. Is there
information
demonstrating that
the method system
suitability tests and
controls as specified
in the SMPR worked
appropriately and as
expected? If no,
please specify.
yes
3. Based on the
supporting
information, is the
method written
clearly and
concisely? If no,
please specify the
needed revisions.
yes
4. Based on the
supporting
information, what
are the pros/strengths
of the method?
the pros is the potential of the MS/MS based
method to detect along one run several allergens
such as egg, milk, hazelnut, peanut with possible
inclusion of other nuts according to some
preliminary data shown. Also another advantage is
the sharp protocol required for sample preparation.
5. Based on the
supporting
information, what
are the
cons/weaknesses of
the method?
sensitivity of the method should be better
investigated and also recovery of the food matrices
depending on the type of inclusion done whether
spiked or incurred.
6. Any general
comments about the
method?
The method has been definitely improved and
enriched with additional details and info also thanks
to other experiments carried out according to what
raised by the reviewers. I would approve the
method only if calculation of recovery is properly
done (also detailing clearly if it refers to the spiked
or incurred food samples; and if it is done according
to what reported in the guidance namely n=7
independent analysis for each concentration level).
Most importantly I would require to calculate in a
correct way the MDL and MQL that is a very