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Challenges ‐ 10 species currently described
Many demonstrate host specificity
Existing Methods
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Foster et al. 2008 SNP‐based real‐time PCR assays
f
ll
j B ll l d
or a ma or ruce a c a es
•
Fretin et al. 2008 SNP‐based real‐time PCR assays
for B. suis (137 field strains)
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Hinić et al. 2008 Conventional and real‐time PCR
assays for multiple Brucella species
•
Lopez‐Goñi et al. 2016 Multiplex “Bruce‐ladder”
for all known species
•
Scholl et al., 2010 Multiplex PCR assay for all
described species
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Yu and Nielsen, 2010 Croatian Med J 51 review