131 / 226
Brucellosis in the Greater Yellowstone Area
•
What is the real potential for
interspecies transmission
between large ungulates and
cattle in the Intermountain
West?
Can rapid accurate molecular
•
,
diagnostics improve
management and minimize
impacts to wildlife?
Real-time PCR assay for
B. abortus
Newby et al. 2003.
Appl. Environ. Microbiol
. 69
•
B. abortus
specific (tested against
panel of over 100 strains)
•
7.5 fg limit of detection (ca. 2 genomic
copies)
•
Semi‐quantitative nature of real‐time
PCR permits estimation of bacterial
load in samples
•
Detection in 15‐30 minutes
•
No sample prep necessary in some
cases
•
Hybridization probes allow
discrimination of amplicons based on
post‐amplification melt curves
(potential to identify S19 and RB51
without multiplexing)
•
New TaqMan assay developed for
other instruments (15 fg LOD)