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Background
•
Confirmation by isolation remains the gold
standard, in spite of aerosol hazard and low
infectious dose
•
Cultivation often includes antibiotics and
antibiotic dyes (thionin, basic fuchsin) to
counterselect against other microbes
•
Select agent regulations complicate this
process! Proposed rule changes out for
comment
Background
Genome-based Brucella taxonomy
Bohlin et al., 2010. BMC Evol. Biol. 10:249