Methods Reviewed by OMB in 2014

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814 Bird et al.: Journal of AOAC International Vol. 96, No. 4, 2013
Table 2013.01B. Summary of results for the detection of Salmonella spp. in raw ground beef (375 g)
Methoda
VIDAS SPT with traditional confirmation on
BGSA and XLT4
VIDAS SPT with traditional confirmation on
IBISA and ASAPb
VIDAS SPT with alternative confirmation on
IBISA and ASAPc
Inoculation level Uninoculated Low High Uninoculated Low High Uninoculated Low High
Candidate
presumptive
positive/total
samples
analyzed
0/132 58/131 130/132 0/132 58/131 130/132 0/132 57/131 130/132
Candidate
presumptive
POD (CP)
0.00 (0.00,
+0.03)
0.44 (+0.34,
+0.55)
0.98 (+0.95,
+1.00)
0.00 (0.00,
+0.03)
0.44 (+0.34,
+0.55)
0.98 (+0.95,
+1.00)
0.00 (0.00,
+0.03)
0.44 (+0.33,
+0.54)
0.98
(+0.965,
+1.00)
sr
d 0.00 (0.00,
+0.16)
0.49 (+0.43,
+0.52)
0.12 (+0.11,
+0.16)
0.00 (0.00,
+0.16)
0.49 (+0.43,
+0.52)
0.12 (+0.11,
+0.16)
0.00 (0.00,
+0.16)
0.49 (+0.44.
+0.52)
0.12 (+0.11,
+0.16)
sL
e 0.00 (0.00,
+0.16)
0.10 (0.00,
+0.27)
0.00 (0.00,
+0.05)
0.00 (0.00,
+0.16)
0.10 (0.00,
+0.27)
0.00 (0.00,
+0.05)
0.00 (0.00,
+0.16)
0.09 (0.00,
+0.26)
0.00 (0.00,
+0.05)
sR
f 0.00 (0.00,
+0.23)
0.50 (+0.44,
+0.52)
0.12 (+0.11,
+0.14)
0.00 (0.00,
+0.23)
0.50 (+0.44,
+0.52)
0.12 (+0.11,
+0.14)
0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.12 (+0.11,
+0.14)
P-value 1.0000 0.1551 0.5190 1.0000 0.1551 0.5190 1.0000 0.1906 0.5190
Candidate
confirmed
positive/total
samples
analyzed
0/132 58/131 130/132 0/132 59/131 130/132 0/132 58/131 130/132
Candidate
confirmed POD
(CC)
0.00 (0.00,
+0.03)
0.44 (+0.34,
+0.55)
0.98 (+0.95,
+1.00)
0.00 (0.00,
+0.03)
0.45 (+0.35,
+0.55)
0.98 (+0.95,
+1.00)
0.00 (0.00,
+0.03)
0.44 (+0.34,
+0.55)
0.98 (+0.95,
+1.00)
sr 0.00 (0.00,
+0.16)
0.49 (+0.43,
+0.52)
0.12 (+0.11,
+0.16)
0.00 (0.00,
+0.16)
0.49 (+0.44,
+0.52)
0.12 (+0.11,
+0.16)
0.00 (0.00,
+0.16)
0.49 (+0.43,
+0.52)
0.12 (+0.11,
+0.16)
sL 0.00 (0.00,
+0.16)
0.10 (0.00,
+0.27)
0.00 (0.00,
+0.05)
0.00 (0.00,
+0.16)
0.09 (0.00,
+0.25)
0.00 (0.00,
+0.05)
0.00 (0.00,
+0.16)
0.10 (0.00,
+0.27)
0.00 (0.00,
+0.05)
sR 0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.12 (0.11,
+0.14)
0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.12 (+0.11,
+0.14)
0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.12 (+0.11,
+0.14)
P -value 1.0000 0.1551 0.5190 1.0000 0.2060 0.5190 1.0000 0.1551 0.5190
Positive
reference
samples/total
samples
analyzed
0/132 57/132 132/132 0/132 57/132 132/132 0/132 54/132 131/132
Reference POD 0.00 (0.00,
+0.03)
0.43 (+0.35,
+0.52)
1.00 (+0.97,
+1.00)
0.00 (0.00,
+0.03)
0.43 (+0.35,
+0.52)
1.00 (+0.97,
+1.00)
0.00 (0.00,
+0.03)
0.41 (+0.32,
+0.50)
0.99 (+0.96,
+1.00)
sr 0.00 (0.00,
+0.16)
0.50 (+0.45,
+0.52)
0.00 (0.00,
+0.17)
0.00 (0.00,
+0.16)
0.50 (+0.45,
+0.52)
0.00 (0.00,
+0.17)
0.00 (0.00,
+0.16)
0.49 (+0.44,
+0.52)
0.09 (+0.08,
+0.16)
sL 0.00 (0.00,
+0.16)
0.00 (0.00,
+0.18)
0.00 (0.00,
+0.17)
0.00 (0.00,
+0.16)
0.00 (0.00,
+0.18)
0.00 (0.00,
+0.17)
0.00 (0.00,
+0.16)
0.05 (0.00,
+0.22)
0.00 (0.00,
+0.04)
sR 0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.00 (0.00,
+0.23)
0.00 (0.00,
+0.23)
0.50 (+0.45,
+0.52)
0.00 (0.00,
+0.23)
0.00 (0.00,
+0.23)
0.49 (+0.44,
+0.52)
0.09 (+0.08,
+0.10)
P -value 1.0000 0.6261 1.0000 1.0000 0.6261 1.0000 1.0000 0.3313 0.4338
dLPOD (C vs R) 0.00 (–0.03,
+0.03)
0.01 (–0.12,
+0.15)
–0.02 (–0.05,
+0.02)
0.00 (–0.03,
+0.03)
0.02 (–0.18,
+0.22)
–0.02 (–0.05,
+0.02)
0.00 (–0.03,
+0.03)
0.03 (–0.18,
+0.24)
–0.01 (–0.05,
+0.03)
dLPOD (CP vs
CC)
0.00 (–0.03,
+0.03)
0.00 (–0.15,
+0.15)
0.00 (–0.04,
+0.04)
0.00 (–0.03,
+0.03)
–0.01 (–0.15,
+0.14)
0.00 (–0.04,
+0.04)
0.00 (–0.03,
+0.03)
–0.01 (–0.21,
+0.23)
0.00 (–0.04,
+0.04)
aResults include 95% confidence intervals.
bTraditional confirmation on ASAP/IBISA = secondary enrichments streaked onto IBISA and ASAP.
cAlternative confirmation = direct streak of the primary enrichment onto IBISA and ASAP.
dRepeatability standard deviation.
eAmong-laboratory standard deviation.
fReproducibility standard deviation.
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