810
B
ird
et al
.
:
J
ournal of
AOAC I
nternational
V
ol
. 96, N
o
. 4, 2013
Table 2013.01A. Summary of results for the detection of
Salmonella
spp. in
raw ground beef (25 g)
Method
a
VIDAS SPT with traditional confirmation on BGSA and XLT4
VIDAS SPT with traditional confirmation on IBISA and ASAP
b
VIDAS SPT with alternative confirmation on IBISA and
ASAP
c
Inoculation level
Uninoculated
Low
High
Uninoculated
Low
High
Uninoculated
Low
High
Candidate presumptive positive/total samples analyzed
0/144
144/144
144/144
0/144
144/144
144/144
0/144
144/144
144/144
Candidate presumptive POD (CP)
0.00
(0.00, +0.03)
1.00
(+0.97, +1.00)
1.00
(+0.97, +1.00)
0.00
(0.00, +0.03)
1.00
(+0.97, +1.00)
1.00
(+0.97, +1.00)
0.00
(0.00, +0.03)
1.00
(+0.97, +1.00)
1.00
(+0.97, +1.00)
s
r
d
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00 (0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
s
L
e
0.00
(0.00 +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
s
R
f
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
P
-value
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
1.0000
Candidate confirmed positive/total samples analyzed
0/144
143/144
144/144
0/144
143/144
144/144
0/144
143/144
144/144
Candidate confirmed POD (CC)
0.00
(0.00, +0.03)
0.99
(+0.96, +1.00)
1.00
(+0.97, +1.00)
0.00
(0.00, +0.03)
0.99
(+0.96, +1.00)
1.00
(+0.97, +1.00)
0.00
(0.00, +0.03)
0.99
(+0.96, +1.00)
1.00
(+0.97, +1.00)
s
r
0.00
(0.00, +0.16)
0.08
(+0.07, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.08
(+0.07, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.08
(+0.07, +0.16)
0.00
(0.00, +0.16)
s
L
0.00
(0.00, +0.16)
0.00
(0.00, +0.03)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.03)
0.00
(0.00, +0.16)
0.00
(0.00, +0.16)
0.00
(0.00, +0.03)
0.00
(0.00, +0.16)
s
R
0.00
(0.00, +0.22)
0.08
(+0.08, +0.10)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.08
(+0.08, +0.10)
0.00
(0.00, +0.22)
0.00
(0.00, +0.22)
0.08
(+0.08, +0.10)
0.00
(0.00, +0.22)
P
-value
1.0000
0.4368
1.0000
1.0000
0.4368
1.0000
1.0000
0.4368
1.0000
Positive reference samples/total samples analyzed
0/144
84/144
138/144
0/144
84/144
138/144
0/144
84/144
138/144
Reference POD
0.00
(0.00, +0.03)
0.58
(+0.50, +0.67)
0.96
(+0.91, +0.98)
0.00
(0.00, +0.03)
0.58
(+0.50, +0.67)
0.96
(+0.91, +0.98)
0.00
(0.00, +0.03)
0.58
(+0.50, +0.67)
0.96
(+0.91, +0.98)
s
r
0.00
(0.00, +0.16)
0.50
(+0.45, +0.52)
0.19
(+0.17, +0.22)
0.00
(0.00, +0.16)
0.50
(+0.45, +0.52)
0.19
(+0.17, +0.22)
0.00
(0.00, +0.16)
0.50
(+0.45, +0.52)
0.19
(+0.17, +0.22)
s
L
0.00
(0.00, +0.16)
0.00
(0.00, +0.18)
0.06
(+0.02, +0.13)
0.00
(0.00, +0.16)
0.00
(0.00, +0.18)
0.06
(+0.02, +0.13)
0.00
(0.00, +0.16)
0.00
(0.00, +0.18)
0.06
(+0.02, +0.13)
s
R
0.00
(0.00, +0.22)
0.50
(+0.45, +0.52)
0.20
(+0.18, +0.24)
0.00
(0.00, +0.22)
0.50
(+0.45, +0.52)
0.20
(+0.18, +0.24)
0.00
(0.00, +0.22)
0.50 (+0.45, +0.52)
0.20
(+0.18, +0.24)
P
-value
1.0000
0.6298
0.0179
1.0000
0.6298
0.0179
1.0000
0.6298
0.0179
dLPOD (candidate vs reference)
0.00
(–0.03, +0.03)
0.41
(+0.32, +0.49)
0.04
(0.01, +0.09)
0.00
(–0.03, +0.03)
0.41
(+0.32, +0.49)
0.04
(+0.01, +0.09)
0.00
(–0.03, +0.03)
0.41
(+0.32, +0.49)
0.04
(+0.01, +0.09)
dLPOD (candidate presumptive vs candidate confirmed)
0.00
(–0.03, +0.03)
0.01
(–0.02, +0.04)
0.00
(–0.03, +0.03)
0.00
(–0.03, +0.03)
0.01
(–0.02, +0.04)
0.00
(–0.03, +0.03)
0.00
(–0.03, +0.03)
0.01
(–0.02, +0.04)
0.00
(–0.03, +0.03)
a
Results include 95% confidence intervals.
b
Traditional confirmation on ASAP/IBISA = secondary enrichments streaked onto IBISA and ASAP.
c
Alternative confirmation = direct streak of the primary enrichment onto IBISA and ASAP.
d
Repeatability standard deviation.
e
Among-laboratory standard deviation.
f
Reproducibility standard deviation.
6