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ABBOTT NUTRITION DIVISION

TITLE

PAGE NO.

Determination of Biotin in Infant, Pediatric and Adult

Nutritionals by HPLC and Fluorescence Detection

4 of 11

j)

pH Meter

k)

Disposable transfer pipettes

l)

Reagent reservoirs: assorted sizes

m)

Volumetric flasks: Class A, assorted sizes

n)

Volumetric pipettes: Class A, assorted sizes

o)

Weighing boats or equivalent

p)

Yellow lights or yellow shields with a cutoff of 385 nm

q)

Microcentrifuge

r)

Microcentrifuge tubes: 2 mL

s)

Magnetic stirring plate

t)

Magnetic stirring bars

D.

CHEMICAL, STANDARD AND SOLUTION

1.

Chemicals/Standards

Unless otherwise noted, the following chemicals should be stored at room temperature. Their -

expiration date is one year after the date they are opened. Upon expiration, the chemicals should be

either discarded or re-evaluated. Discontinue use of any chemicals or solutions whenever indications of

contamination, chemical degradation, or changes in concentration are evident

.

a)

Biotin Standard: USP current official lot, reference standard stored as indicated on label. Store at

room temperature unless otherwise stated. Regardless of expiration date, a standard solution

should be discarded if it is suspected to contain chemical or micro biological contamination,

chemical degradation products, or of a change in concentration is evident.

b) Ethanol (Reagent Alcohol): ACS or equivalent

c) Streptavidin fluorescein conjugate: 1mg/mL, GeneTex Streptavidin-FITC, CAT# GTX30950 or

AnaSpec Streptavidin-FITC, CAT# AS-60659-FITC, or equivalent. Store in freezer.

d)

Laboratory water

e)

Methanol: HPLC grade or equivalent

f)

pH Meter buffer solutions for pH meter standardization

g)

Meta-phosphoric acid: ACS or equivalent

j) Sodium phosphate monobasic: ACS or equivalent

k) Sodium phosphate dibasic: ACS or equivalent

2.

Solutions

a)

0.2 M Sodium Phosphate Monobasic (SPM)

Bio-03 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE