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ABBOTT NUTRITION DIVISION
TITLE
PAGE NO.
Determination of Biotin in Infant, Pediatric and Adult
Nutritionals by HPLC and Fluorescence Detection
4 of 11
j)
pH Meter
k)
Disposable transfer pipettes
l)
Reagent reservoirs: assorted sizes
m)
Volumetric flasks: Class A, assorted sizes
n)
Volumetric pipettes: Class A, assorted sizes
o)
Weighing boats or equivalent
p)
Yellow lights or yellow shields with a cutoff of 385 nm
q)
Microcentrifuge
r)
Microcentrifuge tubes: 2 mL
s)
Magnetic stirring plate
t)
Magnetic stirring bars
D.
CHEMICAL, STANDARD AND SOLUTION
1.
Chemicals/Standards
Unless otherwise noted, the following chemicals should be stored at room temperature. Their -
expiration date is one year after the date they are opened. Upon expiration, the chemicals should be
either discarded or re-evaluated. Discontinue use of any chemicals or solutions whenever indications of
contamination, chemical degradation, or changes in concentration are evident
.
a)
Biotin Standard: USP current official lot, reference standard stored as indicated on label. Store at
room temperature unless otherwise stated. Regardless of expiration date, a standard solution
should be discarded if it is suspected to contain chemical or micro biological contamination,
chemical degradation products, or of a change in concentration is evident.
b) Ethanol (Reagent Alcohol): ACS or equivalent
c) Streptavidin fluorescein conjugate: 1mg/mL, GeneTex Streptavidin-FITC, CAT# GTX30950 or
AnaSpec Streptavidin-FITC, CAT# AS-60659-FITC, or equivalent. Store in freezer.
d)
Laboratory water
e)
Methanol: HPLC grade or equivalent
f)
pH Meter buffer solutions for pH meter standardization
g)
Meta-phosphoric acid: ACS or equivalent
j) Sodium phosphate monobasic: ACS or equivalent
k) Sodium phosphate dibasic: ACS or equivalent
2.
Solutions
a)
0.2 M Sodium Phosphate Monobasic (SPM)
Bio-03 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE