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(g)

Calculate the contents of all-

trans

-β-carotene and

cis

isomers of β-carotene in the test samples:

BC

trans

= RF

BC

x (M

A

/M

S

) x (A

BC

/A

A

) x 100

Where BC

trans

= the all-

trans

-β-carotene in the sample (μg/100 g)

RF

BC

= the response factor for all-

trans

-β-carotene

M

A

= the mass of apocarotenal added to the test sample, in μg

M

S

= the sample weight, in g

A

BC

= the peak area of all-

trans

-β-carotene in the sample chromatogram (AU)

A

A

= the peak area of apocarotenal in the sample chromatogram (AU)

BC

cis

= RF

BC

x (M

A

/M

S

) x ([(A

15cisBC

x 1.4) + (A

13cisBC

x 1.2) + A

9cisBC

+ A

XcisBC

]/A

A

) x 100

Where BC

cis

= the

cis

isomers of β-carotene in the sample (μg/100 g)

RF

BC

= the response factor for all-

trans

-β-carotene

M

A

= the mass of apocarotenal added to the test sample, in μg

M

S

= the sample weight, in g

A

BC

= the peak area of all-

trans

-β-carotene in the sample chromatogram (AU)

A

15cisBC

= the peak area of 15-

cis

-β-carotene in the sample chromatogram (AU)

A

13cisBC

= the peak area of 13-

cis

-β-carotene in the sample chromatogram (AU)

A

9cisBC

= the peak area of 9-

cis

-β-carotene in the sample chromatogram (AU)

A

XcisBC

= the peak area of unidentified

cis

isomers of β-carotene in the sample

chromatogram (AU)

A

A

= the peak area of apocarotenal in the sample chromatogram (AU)

Carot-02 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE