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Table 1. Calibration error for each carotenoid.

all-

trans

-Lutein

all-

trans

-β-Carotene

Apocarotenal

Conc

(μg/100 mL)

Residual

Conc

(μg/100 mL)

Residual

Conc

(μg/100 mL)

Residual

185.6

0.427848006

244.0

-1.561049661

257.0

1.663495109

92.8

1.169099574

122.0

4.765184349

128.5

-1.823403562

37.1

-4.622149486

48.8

-3.505075245

51.4

-3.373542764

18.6

1.855899172

24.4

-1.581828442

25.7

-1.846922498

3.71

-4.881101079

4.88

1.882768999

5.14

5.380373715

C.

Accuracy – Accuracy was determined by spike and recovery from SRM 1849a. SRM 1849a is not

fortified with lutein or β-carotene, but contains very low levels of β-carotene.

Table 2. Recovery of lutein and β-carotene spiked into SRM 1849a (n=3). Background levels of β-

carotene were taken into account.

all-

trans

-Lutein all-

trans

-β-Carotene

Spike level (μg/100 g as fed) 12.9

1.29

15.3

1.53

Avg recovery

99.0% 100.2%

101.9%

96.5%

%RSD

0.05% 0.18%

0.51%

0.62%

D.

Precision

Table 3. Repeatability of milk-based infant formula from SPIFAN sample set (n=3).

cis

-Lutein all-

trans

-Lutein

cis

-β-Carotene all-

trans

-β-Carotene

Avg (μg/100 g as fed)

ND

4.5

6.7

13.6

%RSD

n/a

0.27%

0.68%

0.29%

Table 4. Repeatability of PN soy-based infant formula (n=3).

cis

-Lutein all-

trans

-Lutein

cis

-β-Carotene all-

trans

-β-Carotene

Avg (μg/100 g as fed)

ND

4.1

7.3

15.2

%RSD

n/a

0.58%

1.04%

0.21%

E.

LOQ – Based on spike and recovery data a LOQ of 1.3 μg/100 g was observed for lutein and 1.5

μg/100 g for β-carotene.

F.

References

(a)

Aman et al. (2005).

Food Chem

. 92: 753-763.

(b)

Craft and Soares (1992).

J. Agric. Food Chem

. 40: 431-434.

(c)

Granado et al. (2001).

J. Food Comp. Anal

. 14: 479-489.

(d)

Marx et al. (2000).

Food Chem

. 70: 403-408.

(e)

Müller et al. (2008).

J. AOAC Int

. 91(6): 1284-1297.

(f)

Puspitasari-Nienaber et al. (2002).

JAOCS

79(7): 633-640.

(g)

Szpylka and DeVries (2005).

J. AOAC Int

. 88(5): 1279-1291.

(h)

The United States Pharmacopeial Convention (2014). Revision Bulletin - Beta Carotene.

(i)

Waysek et al. (2010).

J. AOAC Int

. 93(2): 499-509.

Carot-02 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE