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Combined AOAC 1
st
action methods 2015.07 and 2015.08
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Chloride in Milk, Milk Powder, Whey Powder,
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Infant Formula and Adult Nutritionals
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Potentiometric titration method.
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For AOAC / ISO / IDF MLT study
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(
Applicable to the determination of chloride in milk, milk powder, whey powder, infant formula
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and adult nutritionals by potentiometry, with an analytical range of 0.35 to 1060 mg
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chloride/100g reconstituted product, or ready-to-feed liquids).
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Caution: Consult material safety data sheets for all substances that are required and considered
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hazardous. Follow all laboratory safety precautions and wear proper personal
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protective equipment.
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A. Principle
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Reconstitute powder samples by dissolving 25 g powder sample in 200 g warm water (40 ° C),
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while ready-to-feed products (RTF) are used as-is. Precipitate proteins by adding precipitation
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reagents I and II and centrifuge. Acidify the supernatant with nitric acid solution. Titrate chloride
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ions against standardized silver nitrate solution, 0.1 M, potentiometrically using a silver
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electrode to detect the end point.
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2015.07/2015.08 (February 2016)
Chlor-02/Chlor-04
FOR ERP USE ONLY
DO NOT DISTRIBUTE