418 / 670
Experimental
A. Apparatus
(a)
LC system
.
–
Prominence (Shimadzu, Kyoto, Japan) or equivalent.
(b)
MS/MS system
.
–
API 4000 with Electrospray Ionization (ESI) (ABSciex, Framingham, MA)
or equivalent.
(c)
Mass Spectrometry Software
.
–
Analyst (ABSciex) or equivalent.
(d)
Analytical column
.
–
Zorbax 300-SCX, 3.0 x 50 mm, 5 μm (Agilent, Santa Clara, CA) or
equivalent.
(e)
Microwave
.
–
MARS6 (CEM, Mathews, NC) or equivalent.
(f)
Microwave turntable, liner, and cap
.
–
MARSXpress, 55 mL PFA Teflon®, 40 position
(CEM or equivalent).
(g)
Vortex mixer.
–
(VWR, West Chester, PA) or equivalent.
(h)
Analytical balances.
–
Model CPA225D (Sartorius, Goettingen, Germany) or equivalent.
(i)
Horizontal shaker.
–
Model 6010 (Eberbach, Ann Arbor, MI) or equivalent.
(j)
Magnetic stir plate.
–
Model PC-420D (Corning, Corning, NY) or equivalent.
(k)
Positive displacement pipets.
–
Microman, various sizes (Gilson, Middleton, WI) or
equivalent.
(l)
Repeater positive displacement pipet
.
–
Repeater Plus (Eppendorf, Hamburg, Germany) or
equivalent.
(m)
Polypropylene tubes
.
–
Digitube, assorted sizes (SCP Science, Montreal, Canada) or
equivalent.
(n)
Mobile phase containers
.
–
2 L glass (VWR) or equivalent.
(o)
Syringe filters
.
–
0.45 μm PTFE and GHP (Pall, Plano, TX) or equivalent.
Chol-08/Carn-07 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE