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Experimental

A. Apparatus

(a)

LC system

.

Prominence (Shimadzu, Kyoto, Japan) or equivalent.

(b)

MS/MS system

.

API 4000 with Electrospray Ionization (ESI) (ABSciex, Framingham, MA)

or equivalent.

(c)

Mass Spectrometry Software

.

Analyst (ABSciex) or equivalent.

(d)

Analytical column

.

Zorbax 300-SCX, 3.0 x 50 mm, 5 μm (Agilent, Santa Clara, CA) or

equivalent.

(e)

Microwave

.

MARS6 (CEM, Mathews, NC) or equivalent.

(f)

Microwave turntable, liner, and cap

.

MARSXpress, 55 mL PFA Teflon®, 40 position

(CEM or equivalent).

(g)

Vortex mixer.

(VWR, West Chester, PA) or equivalent.

(h)

Analytical balances.

Model CPA225D (Sartorius, Goettingen, Germany) or equivalent.

(i)

Horizontal shaker.

Model 6010 (Eberbach, Ann Arbor, MI) or equivalent.

(j)

Magnetic stir plate.

Model PC-420D (Corning, Corning, NY) or equivalent.

(k)

Positive displacement pipets.

Microman, various sizes (Gilson, Middleton, WI) or

equivalent.

(l)

Repeater positive displacement pipet

.

Repeater Plus (Eppendorf, Hamburg, Germany) or

equivalent.

(m)

Polypropylene tubes

.

Digitube, assorted sizes (SCP Science, Montreal, Canada) or

equivalent.

(n)

Mobile phase containers

.

2 L glass (VWR) or equivalent.

(o)

Syringe filters

.

0.45 μm PTFE and GHP (Pall, Plano, TX) or equivalent.

Chol-08/Carn-07 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE