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(p)

Disposable syringes

.

3 mL (BD Biosciences, Franklin Lakes, NJ) or equivalent.

(q)

Graduated cylinders

.

Assorted sizes (VWR) or equivalent.

(r)

Magnetic stir bars

.

7.9 x 50 mm (VWR) or equivalent.

(s)

Autosampler vials/caps

.

1.5 mL silanized crimp top (VWR) or equivalent.

(t)

Microcentrifuge tubes

.

1.5 mL polypropylene (VWR) or equivalent.

(u)

Bottle top dispenser

.

5 mL acid resistant (Brand, Essex, CT) or equivalent.

(v)

Desiccator

.

glass (VWR) or equivalent.

Note

: Non-specific binding can occur with these analytes when using glassware, so plasticware

should be used at all times for standard/sample preparation. All laboratory plasticware should be

single-use whenever possible. Positive displacement pipets are also mandatory for pipeting to

avoid contamination and for accuracy with organic solvents.

B. Chemicals and Reagents

(a)

Water

.

Optima MS grade (Thermo Fisher Scientific, Waltham, MA) or equivalent.

(b)

Acetonitrile

.

Optima MS grade (Thermo Fisher Scientific) or equivalent.

(c)

Ammonium formate

.

Optima MS grade (Thermo Fisher Scientific) or equivalent.

(d)

Formic acid

.

Optima MS grade (Thermo Fisher Scientific) or equivalent.

(e)

Nitric acid

.

70% w/w, ACS grade (Avantor, Center Valley, PA) or equivalent.

(f)

Isopropanol

.

Optima MS grade (Thermo Fisher Scientific) or equivalent.

(g)

Desiccant

.

(VWR) or equivalent.

(h)

Reference Standard

.

L-Carnitine (USP, Rockville, MD) or equivalent.

(i)

Reference Standard

.

Choline Bitartrate (TCI, Tokyo, Japan) or equivalent.

(j)

Reference Internal Standard

.

L-Carnitine-d3 HCl (CDN Isotopes, Pointe Claire, Québec,

Canada) or equivalent.

Chol-08/Carn-07 (February 2016)

FOR ERP USE ONLY

DO NOT DISTRIBUTE