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10

Table 3 Results of method validation parameters.

Folic acid

5ͲMTHF

Linearity range (μg/100g)

0.31 – 140 1.6Ͳ140

LOD (μg/100g)

0.14

0.75

LOQ (μg/100g)

0.31

1.6

Recovery (%)

85

95

RSD repeatability (%)

8

8

RSD reproducibility(%)

6

6

Appendix

Table 1 Mean FA and 5ͲMTHF (± sd ) in samples of SRM 1849a

SRM 1849a

FA (μg/g)

5ͲMTHF (μg/g)

Certified

2.293 ± 0.062

0.084 ± 0.003

Analysed (Heat only)

2.784 ± 0.130

0.130 ± 0.016

Analysed (TriͲenzyme)

3.913 ± 0.090

0.195 ± 0.019

Table 2 Mean analysed 5ͲMTHF concentrations are compared with the certified value of BCR 485

mixed vegetables

Treatment

Analysed Mean ± sd (μg/g)

Certified mean ± sd (μg/g)

Heat only

1.73 ± 0.040

2.14 ± 0.42

Heat and rat serum

1.61 ± 0.0052

2.14 ± 0.42

Note: The method used here was developed based on commercially available formulae in

Australia.

The method needs some further work to ruggedize it for the high fat / high protein spifan

matrices, as ion counts achieved with these matrices indicate that we are not yet achieving the

recoveries seen with Australian commercial formula samples.

Fol-20 (February 2016)

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