486 / 670
10
Table 3 Results of method validation parameters.
Folic acid
5ͲMTHF
Linearity range (μg/100g)
0.31 – 140 1.6Ͳ140
LOD (μg/100g)
0.14
0.75
LOQ (μg/100g)
0.31
1.6
Recovery (%)
85
95
RSD repeatability (%)
8
8
RSD reproducibility(%)
6
6
Appendix
Table 1 Mean FA and 5ͲMTHF (± sd ) in samples of SRM 1849a
SRM 1849a
FA (μg/g)
5ͲMTHF (μg/g)
Certified
2.293 ± 0.062
0.084 ± 0.003
Analysed (Heat only)
2.784 ± 0.130
0.130 ± 0.016
Analysed (TriͲenzyme)
3.913 ± 0.090
0.195 ± 0.019
Table 2 Mean analysed 5ͲMTHF concentrations are compared with the certified value of BCR 485
mixed vegetables
Treatment
Analysed Mean ± sd (μg/g)
Certified mean ± sd (μg/g)
Heat only
1.73 ± 0.040
2.14 ± 0.42
Heat and rat serum
1.61 ± 0.0052
2.14 ± 0.42
Note: The method used here was developed based on commercially available formulae in
Australia.
The method needs some further work to ruggedize it for the high fat / high protein spifan
matrices, as ion counts achieved with these matrices indicate that we are not yet achieving the
recoveries seen with Australian commercial formula samples.
Fol-20 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE