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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
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folate recovery analysis was found to be satisfactory in all of the studied SPIFAN matrices.
The grand average of % spike recovery of folate based on all of the SPIFAN samples was found to be
99.3% with a % RSD value of 5.4% (n=45), thus a satisfactory overall spike recovery was obtained
with a good precision of the replicate analysis .
LOD and LOQ of the Method:
The LOD and LOQ of the method were calculated based on replicate (n=7) analysis of one of the
SPIFAN reconstituted beverage sample with very low total folate levels (near detection). Total folate
compounds in the sample were analyzed and total folate calculated (mcg/100g) as sum of the detectable
folate compounds as folic acid. Standard deviation (Stdev) of the replicate analysis of total folate in the
sample was calculated. The LOD was calculated as 3 X Stdev and LOQ as 10X Stdev. The calculated
LOD and LOQ of the method are provided in the following Table 23.
Table 23: LOD and LOQ of the Method
Parameters
Analyte
Values as mcg/100g of
reconstituted
liquid/RTF
LOD
Total Folate
0.127
LOQ
0.425
Values based on replicate (n=7) analysis of samples with folate at
very low level (near detection level). Stdev of the replicate analysis
was calculated. LOD = 3xStdev. LOQ = 10xStdev.
LOD and LOQ required by the folate SMPR are < 0.1 and <0.5 mcg/100g respectively. The LOD and
and LOQ obtained for the method are within the requirements of the folate SMPR.
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE