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Silliker

Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys

29

Table 19: Results of folate contribution by the minor folate compounds in the SPIFAN II samples

obtained by the analysis with and without conjugase treatment. Total folate and Minor Folate

(mcg/100g) calculated as sum of the detected folate compounds as folic acid in reconstitute

beverages/RTF liquid samples. Averages and precision (as % RSD) of replicates analyzed on

separate 3 days (total n=9).

Sample

Matrix

No Conjugase Treatment

Conjugase Treatment

Total

Folates as

FA*

(mcg/100g)

Minor Folate =

Total Folate -

(FA +CH3THF)

mcg/100g

Minor

Folates as

% of Total

Folate

Total Folates

as FA

(mcg/100g)

Minor Folate =

Total Folate -

(FA +CH3THF)

mcg/100g

Minor

Folates as

% of Total

Folate

6

as Beverage

SRM 1849a Infant

/Adult Nutritional

Formula

23.8

<0.4

0.0

25.6

<0.4

0.0

7

as Beverage

Adult Nutritional

Powder Milk

Protein Based

26.3

<0.4

0.0

29.9

<0.4

0.0

8

as Beverage

Infant Formula

powder Partially

Hydrolyzed Milk

Based

13.7

<0.4

0.0

16.1

1.0

6.5

9

as Beverage

Infant Formula

powder Partially

Hydrolyzed Soy

Based

17.2

<0.4

0.0

20.3

2.0

9.7

10

as Beverage

Adult Nutritional

Powder Low Fat

32.6

<0.4

0.0

34.0

<0.4

0.0

11

as Beverage

Child Formula

Powder

17.7

<0.4

0.0

20.4

<0.4

0.0

12

as Beverage

Infant Elemental

Powder

26.4

<0.4

0.0

27.7

<0.4

0.0

13

as Beverage

Infant Formula

powder Milk Based

15.4

<0.4

0.0

17.3

<0.4

0.0

14

as Beverage

Infant Formula

powder Soy Based

16.3

<0.4

0.0

19.5

1.9

9.8

15

as Beverage

Infant Formula RTF

Milk Based (SPIFAN

Control Milk)

15.0

<0.4

0.0

16.5

<0.4

0.0

16

as Beverage

Adult Nutritional

RTF High Protein

47.0

<0.4

0.0

55.4

<0.4

0.0

17

as Beverage

Adult Nutritional

RTF High Fat

57.4

<0.4

0.0

68.1

<0.4

0.0

Minor folate contents in the SPIFAN II samples based on the analysis which did not employ conjugase

treatment during the extraction was found to be negligible and they did not contribute to total folate

found in these samples.

Minor folate contents in the SPIFAN II samples based on the analysis which employed conjugase

during the extraction was found to be negligible in most of the samples. Two of the samples, both of

which are soy based formula were found to have a small amount of minor folate which contributed to

2011.06/Fol-22 (February 2016)

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