527 / 670
Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
27
Table 18: Results of folate analysis in the SPIFAN II samples with and without conjugase
treatment during extraction. Total folate (mcg/100g) calculated as sum of the detected folate
compounds as folic acid in reconstitute beverages/RTF liquid samples. Averages and precision
(as % RSD) of replicates analyzed on separate 3 days (total n=9).
Sample
Matrix
Lot#
Analyzed
AS
Total Folate as Folic Acid
with Conjugase
with no Conjugase
Conjugase
/No
Conjugase
%
Av.
Mcg/100g
Liquid/ RTF*
% RSD
Av.
Mcg/100g
Liquid/ RTF*
% RSD
NIST SRM
1849a**
Infant Formila
-
Powder
225.8
2.7
210.6
1.6
107.2
BCR 485**
Dried Veg
Powder
-
Powder
302.4
2.4
259.2
4.4
116.7
NIST SRM
3233**
Breakfast
Cereal
-
Powder
1405.2
6.1
1233.6
3.3
113.9
LCS**
Breakfast
Cereal
-
Powder
56.7
3.6
39.3
11.1
144.2
6
as Beverage
SRM 1849a
Infnt /Adult
Nutr Form.
LCLC10-b Recd
12/4/15
Reconst.
Liquid
25.6
4.9
23.8
8.8
107.9
7
as Beverage
Adlt Nutr Pwd
Milk Protein
Based
11750017V3
Recd 12/4/15
Reconst.
Liquid
29.9
4.8
26.3
17.3
113.4
8
as Beverage
Inf Form pwd
Partially Hydr
Milk Based
1172572116
Recd 12/4/15
Reconst.
Liquid
16.1
4.2
13.7
8.0
118.0
9
as Beverage
Inf Form pwd
Partially Hydr
Soy Based
117257651Z
Recd 12/4/15
Reconst.
Liquid
20.3
3.7
17.2
11.1
118.1
10
as Beverage
Adult Nutr Pwd
Low Fat
00394RFOO
Recd 12/4/15
Reconst.
Liquid
34.0
3.7
32.6
12.1
104.4
11
as Beverage
Child Form
Pwd
00412RFOO
Recd 12/4/15
Reconst.
Liquid
20.4
2.5
17.7
8.8
115.3
12
as Beverage
Inf Elemental
Pwd
00403RFOO
Recd 12/4/15
Reconst.
Liquid
27.7
3.9
26.4
12.8
104.7
13
as Beverage
Inf Form
powder Milk
Based
D04HTCW
Recd 12/4/15
Reconst.
Liquid
17.3
4.6
15.4
10.2
112.1
14
as Beverage
Inf Form
powder Soy
Based
E29JVLV Recd
12/4/15
Reconst.
Liquid
19.5
3.0
16.3
5.3
119.5
15
as Beverage
Inf Form RTF
Milk Based
(SPIFAN
Control Milk)
Control Milk
EV4H2R Recd
12/4/15
RTF
16.5
4.8
15.0
14.1
110.4
16
as Beverage
Adlt Nutr RTF
High Protn
0073ORFOO
Recd 1/22/16
RTF
55.4
1.4
47.0
3.7
117.9
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE