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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
22
9
Infant Formula Powder
Soy based
E29JVLV
POWDER 25.1765
200
10
Infant Formula RTF Milk
based (SPIFAN Control
Milk)
EV4H2R
LIQUID
-
-
11
Adult Nutritional RTF
High Protein
00730RF00
LIQUID
-
-
12
Adult Nutritional RTF
High Fat
00729RF00
LIQUID
-
-
* All the samples received as powder were reconstituted and then
analyzed for folates
Averages and Precision of folate analysis in SPIFAN II samples using the trienzyme extraction
including conjugase treatment:
Nine beverages prepared from the respective powdered SPIFAN samples and three RTF samples were
analyzed for all the targeted folate compounds. The folate in these samples was extracted using the
trienzyme extraction including the enzyme conjugase as described in the method. Results in the
reconstituted samples were calculated and reported on reconstituted beverage basis (mcg/100g of
reconstituted beverage). The folate compounds in the RTF samples were calculated on as is basis
(mcg/100g of RTF liquid). Each detected folate compound was calculated as folic acid and sum of that
provided total folate as folic acid and reported as mcg/100g. Each sample was analyzed in three
replicates for three separate days. The averages and inter-day repeatability/precision (%RSD)
calculated and are presented in the following Table 16.
Table 16: Results of folate analysis the SPIFAN II samples employing the trienzyme extraction
including conjugase treatment. Total folate (mcg/100g) calculated as sum of the detected folate
compounds as folic acid in reconstitute beverages/RTF liquid samples. Averages and precision
(as % RSD) of replicates analyzed on separate 3 days (total n=9).
Sample
Matrix
Lot#
Analyzed
AS
Total Folate as Folic Acid
n
Repeat
days
#
Av. Mcg/100g
Liquid or RTF*
Stdev
% RSD
NIST SRM
1849a** Infant Formula
-
Powder
225.8
(
229.3 + 6.2)
6.1
2.7
3
1
BCR 485**
Dried
Vegetable
Powder
-
Powder
302.4
(315 + 28)
7.3
2.4
3
1
NIST SRM
3233**
Breakfast
Cereal
-
Powder
1405.2
(1510 + 120)
86.2
6.1
3
1
LCS**
Breakfast
Cereal
-
Powder
56.7
2.1
3.6
3
3
6
as
Beverage
SRM 1849a
Infant /Adult
Nutritional
LCLC10-b
Recd
12/4/15
Reconst.
Liquid
25.6
1.3
4.9
9
3
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE