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Silliker
Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
19
Table 12: Total folate as folic analysis in BCR 485 (dried vegetable powder) over a period of
about 3 months
Vitamers sum as Folic
09/11/12 09/14/12 09/18/12 09/21/12 10/10/12 10/12/12 10/19/12
mcg/g
Mean
2.312
2.465
2.208
2.494
3.018
3.333
2.931
%RSD
3.5%
2.3%
1.0%
3.5%
2.2%
8.0%
1.9%
N =
3
3
3
3
3
3
3
Vitamers sum as Folic
10/26/12 11/02/12 11/07/12 11/13/12 12/27/12 Grand
Grand
SD
mcg/g
Mean
2.922
3.195
2.654
2.422
2.716
2.710
0.339
%RSD
0.1%
2.3%
2.4%
0.6%
3.0%
12.5%
N =
3
2
3
3
6
38
38
Certified Total Folate
Value*
3.15 + 0.28
*BCR Certified Total Folate value as folic acid obtained by microbiological assay (L. rhamnosus)
Method Accuracy Evaluation Repeated:
The method accuracy was repeated again recently on more sensitive equipment (Sciex 5500 LC-
MS/MS system). The results are presented in the following Table 13. The results of this investigation
again help in establishing the accuracy and satisfactory intra- and interday precision of the method.
Table 13: Total folate as folic analysis in BCR 485 (dried vegetable powder) and other reference
materials
Sample
Matrix
Expected
Total Folates
(mcg/g)
Average
Total
Folates
(mcg/g)
Stdev
%RSD
n
days
NIST 1849A
infant formulas
2.293 +/-
0.062
2.826
0.2
6.9
23
8
NIST 3233
cereal
15.1 +/- 1.2
14.642
1.1
7.3
24
8
BCR 485
mixed vegetables
3.15 +/- 0.28
3.05
0.3
10.3
25
9
LCS Material
cereal
-
0.678
0.1
7.4
36
12
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE