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Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys
21
The Analysis of the SPIFAN samples:
Details of Reconstitution of the samples before the analysis:
All the 12 samples received from SPIFAN were analyzed in replicates by the method for the targeted
folate analytes. Every sample was analyzed in triplicates for three days with a total of 9 replicates. All
the powdered samples were reconstituted as ready to feed beverage by taking approximately 25 g of
the powder into 200 mL of water Details of the samples and reconstitution are provided in the
following Table 15.
Table 15 : Reconstitution of the powder samples (for method repeatability/precision evaluation)
before the analysis
Sample
Matrix
Lot #/ID
REC'D
AS
Reconstitution*
Weight
In H
2
O
(mL)
1
SRM 1849a
CLC10-b
POWDER 25.0141
200
2
Adult Nutritional
Powder Milk Protein
based
11750017V3
POWDER 25.0658
200
3
Infant Formula Powder
Partially Hydrolyzed
Milk based
11752572116
POWDER 25.0824
200
4
Infant Formula Powder
Partially Hydrolyzed Soy
based
117257651Z
POWDER 25.0286
200
5
Adult Nutritional
Powder Low Fat
00394RF00
POWDER 25.0571
200
6
Child Formula Powder
00412RF00
POWDER 25.128
200
7
Infant Elemental
Powder
00403RF00
POWDER 25.0423
200
8
Infant Formula Powder
Milk based
D04HTCVV
POWDER 25.7039
200
15
Infant Formula RTF
Milk based (SPIFAN
Control Milk)
EV4H2R
12/4/2015
357621827
For Analysis
16
Adult Nutritional RTF
High Protein
00730RF00
12/4/2015
357621840
For Analysis
Found Spoiled
17
Adult Nutritional RTF
High Fat
00729RF00
12/4/2015
357621876
For Analysis
Found Spoiled
18
Adult Nutritional RTF
High Protein
00730RF00
1/22/2016
For Analysis
Replacement
Sample
19
Adult Nutritional RTF
High Fat
00729RF00
1/22/2016
For Analysis
Replacement
Sample
2011.06/Fol-22 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE