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Silliker

Chem, Res. Center Crete, IL – Report of A Validation of LC-MS/MS Method for Folate Analys

18

(% RSD range = 3.2-18.7%) as well as interday precision (% RSD = 15.6%) of the method over a

period of about 3 months was found to be satisfactory.

Table 10: 5-methyl-THF analysis in BCR 485 (dried vegetable powder) over a period of about 3

months

5-CH3-THF Results 09/11/12 09/14/12 09/18/12 09/21/12 10/10/12 10/12/12 10/19/12

mcg/g

Mean

2.291

2.453

2.179

2.530

2.900

2.881

2.881

%RSD

3.4%

0.9%

1.5%

3.2%

1.7%

-

2.1%

N =

3

3

3

3

3

1

3

5-CH3-THF Results 10/26/12 11/07/12 11/13/12 12/27/12 Grand

Grand

SD

mcg/g

Mean

2.949

2.613

2.465

2.708

2.615

0.249

%RSD

0.4%

2.2%

0.5%

3.2%

9.5

N =

3

3

3

6

35

35

Indicative Value*

2.14 + 0.42

*BCR Indicative Value, is not a certified value obtained by BCR analysis using a LC fluorescence

method and hog kidney conjugase based extraction and SPE clean-up.

Table 11: 5-formyl-THF analysis in BCR 485 (dried vegetable powder) over a period of about 3

months

5-CHO-THF

Results

09/11/12

09/14/12

09/18/12

10/19/12

10/26/12

11/02/12

mcg/g

Mean

0.063

0.070

0.073

0.065

0.059

0.071

%RSD

10.6%

51.1%

12.1%

2.9%

18.7%

12.2%

N =

3

3

3

3

3

3

5-CHO-THF

Results

11/07/12

11/13/12

12/27/12

Grand

Grand SD

mcg/g

Mean

0.075

0.052

0.054

0.063

0.010

%RSD

5.2%

13.0%

3.2%

15.6%

N =

3

3

6

25

25

There is no value provided for 5-formyl-THF for this sample by BCR

Total folate as folic acid results obtained for the BCR 485 (dried vegetable powder) by the method

over a period of about 3 months are presented in Table 12 The results obtained were quite

consistent over period of analysis. Values obtained for total folate as folic acid was about 96% of

the lowest range specified for this reference material. Intraday precision (% RSD range = 2.2-

3.3%) as well as interday precision (% RSD = 12.5%) of the method over a period of about 3

months was found to be satisfactory.

2011.06/Fol-22 (February 2016)

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